HomeFAQsWhich alkaline phosphatase, CIP, Antarctic or rSAP, works best?
FAQ: Which alkaline phosphatase, CIP, Antarctic or rSAP, works best?
rSAP (NEB #M0371) is a superior choice because it combines the advantages of both CIP (NEB #M0290) and
Antarctic Phosphatase (NEB #M0289). rSAP has a very high specific activity comparable to CIP, but CIP cannot
be completely heat-inactivated. rSAP is heat-inactivated in 5 minutes, as is Antarctic Phosphatase, but rSAP is
preferred over Antarctic Phosphatase because it does not require added zinc or any other co-factors. As a result,
rSAP can be added directly to restriction enzyme digests. Additionally, after heat-inactivation of rSAP, it is not
necessary to purify vector DNA prior to the ligation reaction.