SHuffle® express (E. coli B) cells (C3028, C3029, C3030) will grow in minimal media, while SHuffle (E. coli K12) is an auxotroph and will require the addition of Isoleucine and Leucine (50 ug/ml) for growth in minimal media. 100X stock solutions of Ile/Leu can be made by dissolving 5 mg/ml of the amino acids in water and filter sterilizing the solution.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.