FAQ: Can the Core and Holoenzyme be used in PURExpress?

Yes. If your gene has a strong sigma 70 promoter it can be translated in PURExpress by adding the Holoenzyme to the reaction. If your gene is under the control of other sigma factors you should use the core enzyme and the specific sigma factor. NEB has successfully used sigma factor 54 and the core enzyme in PURExpress.
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