We have observed that DNA size and quality does affect the microbiome DNA separation, as does the overall quantity of CpG methylated dinucleotides. For example, normal human fibroblast DNA (IMR-90) is methylated at 4–5% of the genome and is effectively separated by this technique (90% of human DNA is removed). Cancer DNA (HeLa, Jurkat) is less methylated globally at 3%, and enrichment is less efficient. Likewise, if the DNA fragment size is less than 15 Kb, enrichment will be less efficient. The larger and more CpG methylated the host DNA, the better the separation will be.