HomeFAQsHow important is the MBD2-Fc bead to DNA ratio?
FAQ: How important is the MBD2-Fc bead to DNA ratio?
It is vital that the ratio of MBD2-Fc-bound beads to DNA input used is 1 μl of
MBD-Fc-bound beads per 6.25 ng DNA (or 160 μl MBD-Fc-bound beads per
1 μg DNA input). It is permissible to increase the amount of beads relative to
DNA input, e.g. 320 μl of MBD2-Fc-bound magnetic beads for 2 μg DNA input.