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  • FAQ: How important is the MBD2-Fc bead to DNA ratio?

    It is vital that the ratio of MBD2-Fc-bound beads to DNA input used is 1 μl of MBD-Fc-bound beads per 6.25 ng DNA (or 160 μl MBD-Fc-bound beads per 1 μg DNA input). It is permissible to increase the amount of beads relative to DNA input, e.g. 320 μl of MBD2-Fc-bound magnetic beads for 2 μg DNA input.