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  • FAQ: How important is the MBD2-Fc bead to DNA ratio?

    It is vital that the ratio of MBD2-Fc beads to DNA input used is 1 μl of MBD-Fc beads per 6.25 ng DNA. It is OK to scale the reaction down to 20 μl MBD-2 beads and 125 ng input DNA.Similarly, it is permissible to increase the amount of beads relative to DNA input, e.g.320 μl of MBD2-Fc beads for 2 μg DNA.