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  • FAQ: Will SDS inhibit Endo D?

    Yes, Endo D is inhibited by SDS and unlike other endoglycosidases, NP-40 does not counteract the SDS inhibition. Endo D is therefore not recommended for use with NEB’s Glycoprotein Denaturing Buffer which contains both SDS and DTT. The enzymeco is instead sold with a 10X DTT solution for denaturation purposes without SDS.

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    Endo D