FAQ: What is the difference between PNGase F, Endo S and Endo D?

PNGase F cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid and complex oligosaccharides from N-linked glycoproteins. Endo S has a high specificity for removing N-linked glycans within the chitobiose core of native IgG. Whereas, Endo D cleaves within the chitobiose core of paucimannose N-linked glycans from glycoproteins and glycopeptides, with or without extensions in the antennae.

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Endo D
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