HomeFAQsI currently have an old tube of Restriction Enzyme - is it still active in the new buffer?
FAQ: I currently have an old tube of Restriction Enzyme - is it still active in the new buffer?
Provided it is still prior to the product’s expiry date, yes. The new buffers are mostly identical, except that BSA has been added directly to the buffer and DTT has been removed. BSA will not harm the reaction and may even enhance it in some cases. Extensive testing has shown that DTT is not required.
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