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  • FAQ: How do I perform a double digest with an enzyme that comes with the new buffer and an enzyme that comes with the old buffer system?

    We recommend using the Double Digest Finder (available by clicking the Tools and Resources tab at neb.com) and use the buffer it recommends.  For example, you have old buffer NEBuffer 1 with one of the enzymes and CutSmart™ Buffer with the second enzyme.  If Double Digest Finder recommends to use NEBuffer 1.1, then you can use NEBuffer 1 + BSA for the double digest.  Alternatively, if it recommends CutSmart Buffer, then you can simply use the new buffer.

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