FAQ: What is the optimal heat shock time for the 96-well plate format NEB 5-alpha Competent E.coli (NEB #C2987P)?

Both the temperature and the timing of the heat shock step are important and specific to the transformation volume and vessel. Using the 96-well plate provided, 10 seconds at 42°C is optimal. Expect approximately 40% loss in transformation efficiency when heat shocking for 30 seconds.
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    Tips for Successful Transformations with NEB® Competent Cells

    Follow these tips to get superior results.