FAQ: What other materials do I need in addition to the NEBNext First Strand Synthesis Module?

If you are starting from Total RNA, we offer a NEBNext Poly A mRNA Magnetic Isolation Module (E7490) which is compatible with the First Strand Synthesis Module. Fragmentation of the RNA will occur in the First Strand Buffer. If you are following the NEBNext Ultra Directional Workflow, we recommend addition of Actinomycin D to the First Strand Synthesis Reaction to increase strand-specificity. Actinomycin D is available from suppliers including Sigma (#A1410) and this should be dissolved in dimethylsulfoxide [DMSO] as described in the NEBNext product manuals.