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  • FAQ: You have replaced NEBuffer 1 with NEbuffer 1.1, NEBuffer 2 with NEBuffer 2.1, and NEBuffer 3 with 3.1. Where is NEBuffer 4.1?

    When BSA was added and DTT was removed, NEB changed the name of NEBuffer 4 to CutSmart™ Buffer.  We felt that the name better represented the >200 restriction enzymes that are available in this new buffer.  Furthermore, this buffer also works well with many of our downstream cloning enzymes (see chart on neb.com).