FAQ: My enzyme used to come with another NEBuffer™, but CutSmart™ Buffer is now recommended?  Why?

CutSmart Restriction Enzyme Buffer

>210 of NEB's restriction enzymes are 100% active in a single buffer. Learn more about CutSmart® Buffer and why it matters to you.

Reformulation of the NEB buffers has allowed some restriction enzymes to now also work well in CutSmart™ Buffer.  This enabled us to offer >200 restriction enzymes in a single buffer, greatly increasing convenience, especially for double digests.