HomeFAQsWhy is the desired mutation missing from the transformants that I screened?
FAQ: Why is the desired mutation missing from the transformants that I screened?
Although this is not a common result, there are a few known causes for background transformants: 1) excessive plasmid in the PCR, 2) selection against specific DNA structures such as inverted or tandem repeats, and 3) selection against the recombinant protein by the host E. coli cells.
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