New England Biolabs is working diligently to ensure we keep our employees and their families safe, while maintaining our business continuity. Despite a "Stay At Home" advisory being put in place in Massachusetts, USA, we are deemed an essential business, and our manufacturing and distribution teams continue to be fully operational. We are continuing to work with our suppliers and distribution partners to ensure uninterrupted access to our products and technical support.
HomeFAQsWhat is the maximum distance that can be tolerated between substitutions?
FAQ: What is the maximum distance that can be tolerated between substitutions?
Due to the back-to-back primer design, mutations within 100 nucleotides of each other can routinely be made in one step. Note that HPLC or PAGE purification is recommended for any primer greater than 60 nts.
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