HomeFAQsTypically, what percentage of transformants will have the desired mutation incorporated?
FAQ: Typically, what percentage of transformants will have the desired mutation incorporated?
Due to the high-fidelity polymerase and robust KLD treatment step, the frequency of desired mutations is quite high (>90% for most experiments). When undesired mutations are observed, they are typically at the plasmid ligation site.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.