FAQ: What is the tag on PNGase F?

PNGase F is tagged with a chitin binding domain (CBD). The CBD tag allows for removal from a reaction using chitin beads (NEB #S6651) or chitin magnetic beads (NEB #E8036). Note: there is no cleavage site between the PNGase F and the CBD tag to enable removal of this tag from the PNGase F.