PNGase F and Remove-iT® PNGase F are purified from the same source with identical specificity. Remove-iT PNGase F has been expressed with a chitin binding domain (CBD) tag to allow for easy removal of the enzyme from a reaction. The tag does not change or alter the specificity or activity of the enzyme. If used under denaturing conditions, PNGase F and Remove-iT® PNGase F have the same unit concentration. However, Remove-iT® PNGase F is sold with a modified denaturing unit (DTT only, no SDS) to make it mass spec friendly.