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  • FAQ: What are the typical reaction conditions for Remove-iT® Endo S?

    Typical reaction conditions are as follows:

    Optimal incubation times and enzyme concentrations must be determined empirically for a particular substrate.

    1. Combine 100 µg of native IgG, 1 µL of 10X GlycoBuffer 2 and H2O (if necessary) to make a 10 µL total reaction volume.
    2. Add 1 µL Remove-iT® Endo S.
    3.  Incubate reaction at 37°C for 1 hour.

    Note: Reactions may be scaled-up linearly to accommodate larger reaction volumes.