HomeFAQsIs Remove-iT® PNGase F compatible with downstream analysis such as HPLC and Mass Spectrometry?
FAQ: Is Remove-iT® PNGase F compatible with downstream analysis such as HPLC and Mass Spectrometry?
Remove-iT® PNGase F is supplied in a mass spec friendly, glycerol free storage buffer. Glycoprotein Denaturing Buffer (containing SDS and DTT) is not compatible with Mass Spectrometry applications, and often times hard to remove from the reaction. Therefore, the Remove-iT® PNGase F unit definition is based on a partial denaturation protocol using only DTT, with no SDS making the reaction compatible with downstream HPLC and MS analysis.