HomeFAQsWhat are the longest overlaps that can be used with this method?
FAQ: What are the longest overlaps that can be used with this method?
The quantity of 5´ exonuclease in the Gibson Assembly Master Mix and a 15 minute assembly reaction time have been optimized for the assembly of DNA molecules with ≤ 25-bp overlaps. If assembly reaction time is increased to 60 minutes, overlaps up to 40-bp may be used with the Gibson Assembly Cloning Kit.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.