HomeFAQsHow large of a DNA fragment can I assemble?
FAQ: How large of a DNA fragment can I assemble?
Gibson Assembly Cloning Kit has been used to clone a 15 kb DNA fragment into a 5.4 kb plasmid in E. coli, totaling up to 20.4 kb in length. For assembled products greater than 15 kb, NEB recommends NEB 10-beta Competent E. coli (High Efficiency, NEB #C3019) or NEB 10-beta Electrocompetent E. coli (NEB #C3020).
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.