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HomeFAQsCan I use a 15-nt overlap that is entirely composed of His-tag repeats (i.e. CACCACCACCACCAC)?
FAQ: Can I use a 15-nt overlap that is entirely composed of His-tag repeats (i.e. CACCACCACCACCAC)?
No, you must flank the His-tag sequence on both sides with at least 2 nucleotides, that are not part of the His-tag repeating sequence. You should avoid repeating sequences at the end of an overlap.
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