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  • FAQ: Is size selection required and what is the recommended method?

    Yes, we do recommend size selection using AMPure XP beads when making libraries from higher DNA input > 50 ng. A size selection is not recommended if DNA input is less than 50 ng. The Ultra kit is also compatible with other size selection methods, including agarose gel and E-gel. A detailed size selection protocol is included, with the DNA fragment size ranging from 150bp to 500-700bp (final library size 250-800bp).