HomeFAQsIs size selection required and what is the recommended method?
FAQ: Is size selection required and what is the recommended method?
Yes, we do recommend size selection using SPRIselect or AMPure XP beads
when making libraries from DNA inputs > 50 ng. A detailed size
selection protocol is included in the manual, with the DNA fragment size
ranging from 150bp to 500-700bp (final library size 250-800bp). The
Ultra II kit is also compatible with other size selection methods,
including agarose gel and E-gel. A size selection is not recommended if
DNA input is less than or equal to 50 ng.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Sign in to your NEB account
Any items placed in your cart as a guest will be lost upon sign in or leaving the site.