FAQ: Can Klenow Fragment (3'→5' exo-) be used in other NEBuffers, including rCutSmart?

Optimal activity is seen in NEBuffer 2. Klenow Fragment (3'→5' exo-) is active in NEBuffers r1.1, r2.1, r3.1 and rCutSmart, as well as NEBuffers 1-4, when supplemented with dNTPs (not included). It can also be used in rCutsmart Buffer. To see its % functional activity in rCutsmart, and that of other DNA modifying enzymes in the cloning workflow, refer to the Activity of DNA Modifying Enzymes in rCutSmart® Buffer chart.