HomeFAQsCan Klenow Fragment (3'→5' exo-) be used in other NEBuffers, including CutSmart?
FAQ: Can Klenow Fragment (3'→5' exo-) be used in other NEBuffers, including CutSmart?
Optimal activity is seen in NEBuffer 2. Klenow Fragment (3'→5' exo-) is active in NEBuffers 1.1, 2.1, 3.1 and CutSmart, as well as NEBuffers 1-4, when supplemented with dNTPs (not included). It can also be used in Cutsmart Buffer. To see its % functional activity in Cutsmart, and that of other DNA modifying enzymes in the cloning workflow, refer to the Activity of DNA Modifying Enzymes in CutSmart® Buffer chart.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Sign in to your NEB account
Any items placed in your cart as a guest will be lost upon sign in or leaving the site.