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  • FAQ: Can AMPure XP beads used for clean-up steps be left in the PCR reactions?

    No. Please make sure that no trace amounts of AMPure XP beads are carried over into the PCR reactions. The activity of the polymerase used in the NEBNext High-Fidelity 2X PCR Master Mix is affected by the beads, and therefore, sub-optimal performance of the polymerase may be observed.