FAQ: How much endoglycosidase should I use to deglycosylate Fetuin under native conditions?

When Fetuin is not denatured the endoglycosidase may have a more difficult time reaching the cleavage site of the carbohydrate (because of the secondary and tertiary protein structure). Extra enzyme and extended incubation times will help but these values are specific to each protein and must be determined empirically.

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