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  • FAQ: What is the Proteinase K activity in commonly used buffers?

    Buffer: 30 mM Tris HCl, pH 8.0
    Application: Reference
    Relative activity (approx.): 100%

    Buffer: 50mM Tris-HCl, pH 8.0, 1 mM CaCl2, 3 mM DTT, 2.0 M Urea
    Application: Denaturation of proteins
    Relative activity (approx.): 70%

    Buffer: 100 mM Tris-HCl, pH 8.0, 100 mM EDTA, 250mM NaCl, 1% Sarkosyl
    Application: Plant tissue DNA isolation
    Relative activity (approx.): 120%

    Buffer: 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5% SDS
    Application: Bacterial DNA isolation
    Relative activity (approx.): 100%

    Buffer: 10 mM Tris HCl, pH 8.0, 50 mM NaCl, 5 mM EDTA, 1 mM DTT, 0.5% SDS 50 mM Tris-HCl, pH 8.0
    Application: Denaturation of CIP
    Relative activity (approx.): 100%

    Buffer: 50 mM EDTA, 5% Tween 20, 0.5% Triton-X 100, 800 mM GuHCl
    Relative activity (approx.): 300%