• My NEB
  • Print
  • PDF
  • FAQ: Why is the product a smear when visualized on an agarose gel?

    When PCR conditions are not optimal, a smear or high level of background is often observed. Try one or more of the following suggestions:
    • use lower amount of enzyme
    • use 65°C for extension
    • raise annealing temperature
    • try 2-step cycling protocols