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HomeFAQsWhat is the difference between PNGase F, Endo H and O-Glycosidase?
FAQ: What is the difference between PNGase F, Endo H and O-Glycosidase?
PNGase F removes all types of N-linked (Asn linked) glycosylation; high mannose, hybrid, bi, tri, and tetra-antennary. You will choose this enzyme if your goal is to remove all N- linked carbohydrates without regard to type. Endo H removes only high mannose and some hybrid types of N-linked carbohydrates. You would choose this enzyme to more closely determine the type of N-linked glycosylation, or if you know that the protein has a carbohydrate sensitive to Endo H. NEB’s O-Glycosidase will remove desialyated core 1 and core 3 O-linked disaccharides attached to Ser/Thr residues.