Yes. Gaussia and Cypridina are both secreted luciferases, which produce high bioluminescent signal intensity. They oxidize different substrates that do not cross-react with each other. Therefore, Gaussia and Cypridina are an ideal duo for cotransfecting mammalian cells (2,3). Refer to the BioLux Cypridina Luciferase (CLuc) Assay Kits and CLuc expression vectors for more information.