NEB will be closed Thursday, November 23, 2017 and Friday, November 24, 2017. Orders placed will process Monday, November 27, 2017 for delivery on Tuesday, November 28,2017.

FAQ: How should I set up a PCR using the Hot Start Taq 2X Master Mix?

The general guidelines for a 50 µl reaction are:
1X Hot Start Taq Master Mix
0.2 μM each primer
1-1000 pg plasmid or 1-1000 ng genomic template 
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