Home FAQs How should I set up a PCR using the Hot Start Taq 2X Master Mix?

FAQ: How should I set up a PCR using the Hot Start Taq 2X Master Mix?

The general guidelines for a 50 µl reaction are:
1X Hot Start Taq Master Mix
0.2 μM each primer
1-1000 pg plasmid or 1-1000 ng genomic template