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  • FAQ: What does HF® refer to following the name of a restriction enzyme?

    Reduce Star Activity with High-Fidelity Restriction Enzymes

    NEB has engineered HF® enzymes to eliminate star activity. Learn how, and what this means for your digests.

    HF® stands for high fidelity. Many restriction endonucleases are capable of cleaving sequences which are similar but not identical to their defined recognition sequence. This altered or relaxed specificity has been termed star activity. HF restriction endonucleases have been engineered to cleave with higher fidelity than the wild type enzyme, hence exhibiting less star activity. Screens using increased glycerol concentration, increased reaction time and high enzyme concentration were used to identify enzymes that would offer the highest fidelity over a wide range of conditions In addition, all HF enzymes are supplied with CutSmart Buffer as well as a free vial of Purple Loading Dye. Learn more about High Fidelity Restriction Enzymes here.