HomeFAQsWhat conditions are recommended for SNAP-tag labeling in vitro?
FAQ: What conditions are recommended for SNAP-tag labeling in vitro?
The SNAP-tag labeling reaction is tolerant of a wide range of buffers. The requirements of the fusion partner should dictate the buffer selected. The following buffer guidelines are recommended: pH between 5.0 and 10.0, monovalent salts (e.g. sodium chloride) between 50 mM to 250 mM and at least 1 mM DTT. Non-ionic detergents can be added to 0.5% v/v if required, but SDS and other ionic detergents should be avoided entirely because they inhibit the activity of the SNAP-tag. Metal chelating reagents (e.g., EDTA and EGTA) also inhibit SNAP-tag activity and should be avoided.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.