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  • FAQ: Are NEB's endoglycosidases compatible with protease inhibitor cocktails?

    When a protein is denatured it is more susceptible to cleavage by proteases. For this reason a protease cocktail containing the following can be used in a PNGase F, Endo H, O-Glycosidase or Protein Deglycosylation Mix reaction protocol:

    Aprotinin (10 µg/ml final concentration), Benzamidine (1 mM final concentration), Pepstatin (10 µg/ml final concentration), Leupeptin (1 µM final concentration), EGTA (1 mM final concentration), EDTA (1 mM final concentration), PMSF (1 mM final concentration). Note: PMSF is not highly recommended as it has the ability to modify basic residues on glycoprotein substrates.