NEB will be closed Thursday, November 23, 2017 and Friday, November 24, 2017. Orders placed will process Monday, November 27, 2017 for delivery on Tuesday, November 28,2017.

FAQ: How should I set up a PCR using Hot Start Taq DNA Polymerase?

The general guidelines for a 50 µl reaction are:
1.25 units Hot Start Taq DNA Polymerase
200 μM each dNTP
0.2 μM each primer
1-1000 pg plasmid or 1-10000 ng genomic template
1 X Standard Taq Reaction Buffer
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