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HomeFAQsHow should I set up a PCR using Hot Start Taq DNA Polymerase?
FAQ: How should I set up a PCR using Hot Start Taq DNA Polymerase?
The general guidelines for a 50 µl reaction are:
1.25 units Hot Start Taq DNA Polymerase
200 μM each dNTP
0.2 μM each primer
1-1000 pg plasmid or 1-10000 ng genomic template
1 X Standard Taq Reaction Buffer
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