FAQ: How should I set up a PCR using Hot Start Taq DNA Polymerase?

The general guidelines for a 50 µl reaction are:
1.25 units Hot Start Taq DNA Polymerase
200 μM each dNTP
0.2 μM each primer
1-1000 pg plasmid or 1-10000 ng genomic template
1 X Standard Taq Reaction Buffer