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  • FAQ: How should I set up a PCR using Hot Start Taq DNA Polymerase?

    The general guidelines for a 50 µl reaction are:
    1.25 units Hot Start Taq DNA Polymerase
    200 μM each dNTP
    0.2 μM each primer
    1-1000 pg plasmid or 1-10000 ng genomic template
    1 X Standard Taq Reaction Buffer