FAQ: What precautions should be taken while using the mRNA Cap 2'-O-Methyltransferase?

It is important to make sure that RNA being used in the reaction is free from salts and EDTA. It should preferably be re-suspended in nuclease-free water. We strongly recommend using gloves and nuclease-free tubes, pipette tips and reagents to prevent RNase contamination. RNase Inhibitor (NEB# M0314 ) may be used in the reaction to prevent degradation due to contaminating RNases.