Fourth of July Shipping Notice: Orders placed on Friday, July 1, 2022, will be shipped on Tuesday, July 5, 2022, for delivery on Wednesday, July 6, 2022.
HomeFAQsWhat kind of RNA can be methylated using the mRNA Cap 2'-O-Methyltransferase?
FAQ: What kind of RNA can be methylated using the mRNA Cap 2'-O-Methyltransferase?
mRNA Cap 2'-O-Methyltransferase requires capped RNA as substrate. Capped RNA can be prepared via in-vitro transcription (T7 High Yield RNA Synthesis Kit, NEB# E2040) using cap analog (ARCA 3´-O-Me-m7G(5')ppp(5')G RNA Cap Structure Analog, NEB# S1411) or by enzymatic capping using the Vaccinia capping enzyme (NEB# M2080).