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  • FAQ: What should I take into consideration when designing a set of PCR primers?

    1. Avoid complementarity between the primers to prevent primer-dimer formation.
    2. Avoid inverted repeats (self-complementarity).
    3. GC-content of the primer should be around 50%.
    4. Avoid Gs and Cs at the 3'-end of the primers.

    There are many computer programs which will help you to design a primer pair, such as Primer3.