FAQ: What should I take into consideration when designing a set of PCR primers?

1. Avoid complementarity between the primers to prevent primer-dimer formation.
2. Avoid inverted repeats (self-complementarity).
3. GC-content of the primer should be around 50%.
4. Avoid Gs and Cs at the 3'-end of the primers.

There are many computer programs which will help you to design a primer pair, such as Primer3.
Loading Spinner