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  • FAQ: What is the composition of the ThermoPol buffer?

    1X ThermoPol Reaction Buffer: 20 mM Tris-HCl, 10 mM (NH4)2SO4, 10 mM KCl, 2 mM MgSO4, 0.1% Triton®-X-100, pH 8.8 @ 25 °C. Supplied as a 10X concentrated stock. For reactions requiring SNTPs, Deoxynucleotide Solution Mix (NEB# N0447) or Deoxynucleotide Solution Set (NEB# N0446) are available separately.