Home FAQs How should I set up a PCR using the OneTaq® Quick-Load® Master Mixes?

FAQ: How should I set up a PCR using the OneTaq® Quick-Load® Master Mixes?

The general guidelines for a 50 µl reaction are:
1X OneTaq® Quick-Load® Master Mix
0.2 μM each primer
1-1000 pg plasmid or 1-1000 ng genomic template
Denature at 94°C
Extend 1 minute/kb at 68°C