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  • FAQ: What is the composition of the ThermoPol® II (Mg-free) Reaction Buffer?

    1X ThermoPol II Reaction Buffer: 20 mM Tris-HCl, 10 mM (NH4)2SO4, 10 mM KCl, 0.1% Triton® X-100 (pH 8.8 @ 25°C). Supplied as a 10X concentrated stock. For reactions requiring dNTPs, Deoxynucleotide (dNTP) Solution Mix (NEB# N0447) or Deoxynucleotide (dNTP) Solution Set (NEB# N0446) are available separately.