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  • FAQ: Is the Phusion® Hot Start Flex 2X Master Mix a substitute for the discontinued Phusion Flash High-Fidelity PCR Master Mix (F-548S/L)?

    For most applications utilizing the Phusion Flash High-Fidelity PCR Master Mix, Phusion Hot Start Flex 2X Master Mix can be used as a substitute. However, we find that using standard Phusion cycling conditions (not Flash cycling conditions) will give more robust and consistent results.