HomeFAQsWhat is the extension rate when using LongAmp™ Hot Start Taq 2X Master Mix?
FAQ: What is the extension rate when using LongAmp™ Hot Start Taq 2X Master Mix?
We recommend 50 seconds per kb for maximum yields. Extension rates such as 30 seconds per kb can be used for targets up to 4 kb using a 3-step PCR protocol. Shorter extension rates, such as 15 seconds per kb, can be used for targets up to 2 kb using a 3-step PCR protocol on a fast PCR machine.
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