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  • FAQ: How should I set up a PCR using OneTaq® Hot Start DNA Polymerase?

    The general guidelines for a 50 µl reaction are:
    1.25 units OneTaq Hot Start DNA Polymerase
    200 μM each dNTP
    0.2 μM each primer
    1-1000 pg plasmid or 1-1000 ng genomic template
    1 X Standard Buffer (GC Reaction Buffer can be used for GC-rich amplicons)
    Denature at 94°C (no separate activation step is required for the aptamer-based Hot Start enzyme)
    Extend 1 minute/kb at 68°C
    Reactions can be assembled at room temperature.