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  • FAQ: Can OneTaq® DNA Polymerase be used with uracil-containing primers or bisulfite-treated DNA?

    The presence of an archaeal polymerase does not prevent OneTaq® DNA polymerase from using dU-containing DNA as a substrate. OneTaq DNA Polymerase is compatible with USER cloning methods and bisulfite-treated DNA. For protocols employing bisulfite-treated DNA that require a hot start enzyme, we recommend OneTaq® Hot Start DNA Polymerase.