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  • FAQ: Can aberrant RNA be produced when using SP6 RNA Polymerase?

    Aberrant RNA has been noted when the restriction enzyme used to linearize the plasmid downstream of the DNA of interest produces a 3' overhang (1). To avoid this, the plasmid should be cut with a restriction enzyme that leaves a blunt or 5' overhang downstream of the DNA of interest.
    (1) Schenborn, E. T. and Mierendorf, R. C., Jr. (1985) Nucleic Acids Res 13, 6223-6236.