FAQ: Can DNA Polymerase I (E. coli) be used in other NEBuffers, including rCutSmart?

DNA Polymerase I (E. coli) is active in NEBuffers r1.1, r2.1, r3.1 and rCutsmart, as well as NEBuffers 1-4, however, optimal activity is observed in NEBuffer 2. All NEBuffers must be supplemented with dNTPs. To see its % functional activity in rCutsmart, and that of other DNA modifying enzymes in the cloning workflow, refer to the Activity of DNA Modifying Enzymes in rCutSmart® Buffer chart.