Home FAQs If I forget to add the 3’ SR RT Primer 3 before the 5’ ligation can I add it before the reverse transcription step and move forward?

FAQ: If I forget to add the 3’ SR RT Primer 3 before the 5’ ligation can I add it before the reverse transcription step and move forward?

If you forget to add the 3’ SR RT Primer 3 before the 5’ ligation you can add it before reverse transcription and move forward with the protocol. This will however cause a lower yield in PCR (and thus more cycles) and will necessitate size selection to remove adapter-dimer.