NEB will be closed Thursday, November 23, 2017 and Friday, November 24, 2017. Orders placed will process Monday, November 27, 2017 for delivery on Tuesday, November 28,2017.

FAQ: How should I set up a PCR reaction using Q5® Hot Start 2X Master Mix?

The general guidelines for a 50 µl reaction are: 

0.5 μM each primer
2-50 pg plasmid or 50-500 ng genomic template
1X Q5i Hot Start High-Fidelity 2X Master Mix
Denature at 98°C
Extend 20 s/kb at 72°C 

Reactions can be set up at room temperature
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