• My NEB
  • Print
  • PDF
  • FAQ: How should I set up a PCR reaction using Q5® Hot Start 2X Master Mix?

    The general guidelines for a 50 µl reaction are: 

    0.5 μM each primer
    2-50 pg plasmid or 50-500 ng genomic template
    1X Q5i Hot Start High-Fidelity 2X Master Mix
    Denature at 98°C
    Extend 20 s/kb at 72°C 

    Reactions can be set up at room temperature